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Samuel Langhorne Clemens, better known by his pen name of Mark Twain, was an American author and humorist who appears on Mystery Train Island. He wrote the books The Adventures of Tom Sawyer and its sequel Adventures of Huckleberry Finn. At the time of Mystery Train, he was writing Huckleberry Finn.

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Mark Twain is one of the passengers on The John Bull. He was originally a suspect who was thought to be the thief that stole Edison's Motion Capture Device by you until he was proven innocent. He provides some clues throughout your investigation including the info about the mysterious person in the last car. Most of the time, however, he is seen in his passenger cabin suffering from motion sickness and complaining about the coal fumes.

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